Staff at How Hill Trust near Ludham noticed the donation box was missing on Thursday, September 14.
It is the second time this year that the box has been reported stolen after a theft in April.
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The garden was shut for a few days after the theft earlier this year during which time the charity replaced the box with a sturdier one mounted on a metal frame and set in concrete.
How Hill Trust director Simon Partridge said: “It is disappointing to have the donation box stolen from our charity.
“Donations are so important to us – raising £4000-6000 in a typical year to help fund our work.
“We are not sure how much money was inside but it must have taken a lot of time and effort to commit the crime.”
He believes somebody dug up the frame and cut off the box using a hacksaw.
Police are asking anyone who may have seen anything suspicious, found the discarded box, or with information to contact Norfolk police.
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